Chapter 6, Ascitic Tumor-bearing Mice

Gu Jun nodded in response to her, it had been three months since they last met, and the squad leader was still as beautiful as ever, but the person he once was was no longer the same

Gu Jun. "Wang Ruoxiang suddenly turned her head and glanced at him, 'Could you move away a bit? You're in the way.'"

He seems unable to breathe, as if he is in a dilapidated laboratory, with centrifuges, microscopes, autoclaves, and various instruments scattered all over the floor, broken test tubes, disordered documents, blurred picture frames, ... the putrid corpses of animals ... . ... Dust has turned everything into a dull color, devoid of any breath of life, only chaos and deathly silence

The modeling of Mouse No. 1 has been successfully completed. Wang Ruoxiang was finally relieved and happy, smiling at the team members: "The ascites contain a large number of S180 tumor cells!"

Indeed, that is Wang Ruoxiang

He began to murmur softly

Cai Zixuan immediately revealed a knowing look; just moments ago, he had claimed not to be concerned about the class monitor, yet now he was eager to join in the excitement. Youth is truly wonderful

Oh. Gu Jun also leaned over to take a look, still understanding what they were doing

"No, I won't." Gu Jun obediently stood aside, but he always felt that Wang Ruoxiang's gaze was unfriendly... Perhaps it was just an illusion

Each of them is full of fighting spirit, and no one cares about how the wealthy and handsome man is doing anymore, even forgetting that he is standing by watching.

Rich and handsome... ... ?

Wang Ruoxiang silently pressed her lips together and then said: "Just stand aside and don't cause any trouble."

Upon hearing her words, the team members immediately became somewhat excited, gathering around to get a clearer view, "Is this the 'frog belly'?" "Impressive."

The class monitor is still single now. Cai Zixuan clearly does not realize how much of a busybody she appears at the moment

Thus, it is first to create, and then to expand. Now that the mice have developed frog-like abdomens, they can "absorb abdominal fluid".

Is this the situation? Gu Jun furrowed his brows in helplessness, thinking it was something significant. Not surprised at all, really. If it weren't her, I would be puzzled.

The twisted corpses on the ground... are utterly unrecognizable as any known animal...

Day 8 of the abdominal cavity implantation of S180 fibrosarcoma in mice. Wang Ruoxiang was focused and serious, gently pressing on the noticeably swollen abdomen of Mouse 1, which let out a series of painful squeaks and struggled incessantly, yet she held it firmly in her hand

The research proposal and the project initiation report were all handled by the team leader. Cai Zixuan couldn't help but exclaim, "Truly worthy of Wang Ruoxiang."

Because if the experiment results in a paper, Wang Ruoxiang will be the first author, while Cai Zixuan and the others can only be listed afterwards

Once the team members have gathered, everyone immediately begins to work

Yuhan, you are responsible for taking 0.2 ml of cells for each needle.

Although Professor Gu is a mentor, he only occasionally finds time to provide guidance. For someone of his stature, focusing on publishing high-scoring SCI articles is his primary concern. Therefore, the Pioneer Cup ultimately depends on the students themselves. The team leader does the most work, yet it is a coveted opportunity that everyone desires.

Suddenly, Gu Jun felt a sharp pain in his head. Could it be an attack from a brainstem tumor

"Gao Jun had to take a leave of absence due to personal matters, but he is back now," Cai Zixuan explained to everyone, introducing Gu Jun: "This is Xu Hai, a familiar face from the clinical medicine program, and also Zhang Haoran from the basic medical sciences program, and He Yuhan from the pharmacy program. The group consists of five people in total.

The allocated funding this time is only 30,000 yuan, which is considerable for an undergraduate research experiment. However, an imported pure Photofrin photosensitizer costs over 10,000 yuan. In order to conduct experiments with multiple types of photosensitizers, Wang Ruoxiang meticulously calculated and saved funds to establish domestic imitation Photofrin group, multiple porphyrin group, ALA group, along with a blank control group, totaling 5 groups of mice

In the conversation, the two took the elevator to the 8th floor, where the laboratory used by their group is located

The reason for first creating ascitic tumor-bearing mice is to obtain a large quantity of highly active S180 tumor cells. It is important to note that the original cell line is quite expensive; one vial is only sufficient for injecting a single mouse, and its activity is very fragile. Moreover, the four treatment groups they established require the cultivation of hundreds of mice

The wealthy and handsome. ... She murmured to herself, recalling a few years ago at the beginning of the school year, when she had a superficial fondness for him ... . ... Ugh ... . Such a person, lazy and gluttonous, with all the vices, a rotten wood that cannot be carved, and mud that cannot be propped up against a wall. ... ... Not even as good as a little mouse! Perhaps only by being taken for dissection research would he make any contribution to medicine.

Wang Ruoxiang is the class monitor, possessing strong abilities, good looks, and a pleasant personality. As a top student goddess, it is no surprise that those pursuing her line up from the teaching building to the administrative building, among them is the wealthy and handsome Jun. His pursuit was nothing short of fervent, but ultimately ended with Wang Ruoxiang's public and stern rejection: "I am sorry, we are not of the same kind, I will not like someone like you."

We are still in the first stage, which is to establish an ascitic tumor-bearing mouse model. We have been cultivating it for a week, and today we will see if it is successful

Here. After entering the building, Cai Zixuan walked ahead, introducing the situation to Gu Jun as he walked.

"Sure." Gu Jun shrugged and walked away, your aura is strong enough that even being one meter away from you can be a hindrance

Yay! Haha. They are all young people, no one cares about the feelings of the little mouse, they cheered, and Cai Zixuan praised: "Taking the first step towards success!"

The title of the experiment conducted by their group is "Research on Photodynamic Therapy of Tumors Using Various Photosensitizers". This study involves subcutaneously implanting tumors in mice, followed by photodynamic therapy using different photosensitizers, and then observing the changes in the tumors.

In a moment of daze, Gu Jun suddenly seemed to understand the meaning of that line of blood characters

At that moment, everyone saw a person walking in, and the laboratory fell silent, leaving only the chirping of the mice.

Cai Zixuan spoke at length, suddenly realizing that Gu Jun had been silent for a while, seemingly frowning at the mention of the word "tumor"

"Indeed, it is the belly of a frog; it is truly large," Cai Zixuan remarked

He saw again that on the wall inside, there was a line of blood writing, in that same mysterious language as on the medicine box, but compared to the standard of printed text, each stroke of that blood writing had a chilling strangeness to it

Cai Zixuan scratched the balding area on his head, truly unsure whether Gu Jun understood what he had just said

Indeed. Cai Zixuan was deeply moved by this statement, his expression reflecting a sense of profound weariness, "All conditioned phenomena are like dreams, illusions, bubbles, and shadows"

Adjust the cell concentration to 1 × 10^7 / ml

Hello everyone. Gu Jun smiled and waved to greet everyone, then looked at Wang Ruoxiang, saying, "Hello, class monitor."

Well, a tall, handsome, and wealthy man, who is still a graduate of an eight-year program, turns out to be "this kind of person"

Well, Gu Jun does not understand this...

At that time, everyone said, thanks to the wealthy and handsome class leader, they were able to believe in love once again

The first step was successful, and the next step is to induce subcutaneous tumors in the mice of the treatment group using these cells, followed by photodynamic therapy after tumor formation

You understand nothing at all

What? Gu Jun felt truly helpless. He wanted to explain but did not know where to start. "Forget it..."

To maintain cell viability, all injections must be completed within one hour. Therefore, they finish with one modeling mouse before moving on to the next one

With a crackling sound, Wang Ruoxiang put on rubber gloves and approached the mouse cage to catch the mice, which had all been marked with dye and numbered. She successfully caught mouse number 1. Cai Zixuan and several others were nearby, responsible for assisting, recording, filming, and taking photographs.

Team leader, the medicine has been prepared!

Is that so? Cai Zixuan decided not to continue discussing the class monitor's matters, glanced at the watch, and said, "Oh, it's almost 8 o'clock, I need to go to the laboratory"

Walking along the corridor, upon reaching the door of the laboratory, Cai Zixuan pushed the door open and entered first, only to find that all the team members were already present, making him the last one to arrive

Eh?

Apart from a cage of model mice, the rest are all blank mice, which Wang Ruoxiang has just purchased from the school's laboratory animal center. Once the modeling is successful, they will be put to use

She stated: "The ascites in Mouse No. 1 continues to increase, it has already reached the level of a frog's abdomen, and its vitality is good; there is no need for euthanasia at this time"

Didn’t he say he dropped out to travel around the world? Didn’t he say he failed? ... Didn’t he say he ...

What in the world can be one hundred percent? Gu Jun lowered his head and covered his face, my friend, I haven't sunk to that level of being a poor student, have I

Cai Zixuan looked around and then said, "The leader of our group is Wang Ruoxiang"

The pain in his brain grew increasingly intense, yet the images became ever clearer, allowing him to discern some indescribable instruments

There are still many tasks to be done, and they continue to be busy, removing the upper clear liquid from the centrifuge tubes, resuspending for counting, adjusting the cell concentration

Zixuan, I have been waiting for you

"Do not look at me like that," Gu Jun said with a self-deprecating smile, "It is all in the past." Indeed, it is all in the past. The wealthy Jun's pursuit of the class monitor was partly out of genuine affection, but even more so out of a desire to win over everyone's goddess. Ultimately, it had little to do with anything profound.

She used a 5ml syringe to puncture the abdominal cavity of mouse number 1, successfully drawing out a full syringe of yellow, clear ascitic fluid, which she then injected into an Eppendorf conical bottom centrifuge tube, and took it to the centrifuge for spinning. Once centrifuged, a large amount of white precipitate could be seen at the bottom of the centrifuge tube, which was precisely the tumor cells

The pain suddenly intensified, and he couldn't help but cry out, "Ah!" The scene before him became hazy, flickering like the shadows of a nightmare

Wang Ruoxiang stood by an experimental table, her short black hair framing a delicate oval face that, despite being bare of makeup, was quite attractive. Her tall figure was complemented by a white lab coat, giving her an air of confidence. Upon seeing him, Wang Ruoxiang slightly furrowed her brows and said, "Gu Jun, hello." Her voice was also pleasant to hear

Gu Jun quietly observed as He Yuhan prepared the cell suspension, Cai Zixuan administered intraperitoneal anesthesia with pentobarbital sodium to the healthy mice, Xu Hai and Zhang Haoran labeled the mice with numbers and depilated their hindquarters, and finally, Wang Ruoxiang injected tumor cells subcutaneously into the right hindquarters of the mice

"Let's go, I also need to head to the laboratory building," Gu Jun said

"Hao Jun, you need not say anything; I understand. Once having experienced the vast ocean, it is difficult to regard other waters as significant. I comprehend this."

Cai Zixuan and the others all pretended not to see it, while Wang Ruoxiang continued with the next step of her operation

Cai Zixuan asked, "Class monitor, is it alright if Hao Jun wants to join us to take a look?"

On the laboratory bench, there is a row of several mouse breeding cages, each containing a group of white Kunming mice. These are the most commonly used experimental animals, known for their docile and small size. At the age of 1 to 1.5 months, they weigh only 18 to 22 grams and have a body length of less than 10 centimeters, making them suitable for experimentation

Although they are both eight-year programs, this is indeed the first time they are conducting such genuine scientific research experiments

The Medical Experiment Building is located in the northwest part of the campus, characterized by a rather unremarkable multi-story structure with white brick walls. Directly across from the entrance lies a dilapidated patch of worn-out concrete. However, inside the building, there are well-equipped laboratories with various instruments and facilities available for research use by faculty and students

Uh, Hao Jun... you know, the success rate of modeling is not one hundred percent

Number 1, Number 2, Number 3, one after another